Leptospira Ag Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Leptospira (LEP Ag) in serum or Urine.
Principle |
Sandwich lateral flow immunochromatographic assay |
Testing time |
5-10 minutes |
Packing content |
-10×foil pouches, each containing one cassette, one pipette and a desiccant
-10×assay buffer tubes
-10×centrifugal tubes
-10×Pipette(10ul)
-Product Manual
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Storage |
Room temperature (2-30°C) |
Caution |
-For best results, please strictly adhere to these instructions.
-All reagents must be at room temperature before running the assay.
-Do not remove test cassette from its pouch until immediately before use.
-Do not reuse the test kit.
-Do not use the test beyond its expiration date marked on the foil pouch.
-The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
-All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states
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【PRINCIPLE】
POCTVET Leptospira Ag Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough LEP antigen in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Dirofilaria immitis antigen in the sample.
【Directions For Use】
-Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.
-Take dog’s blood 1ml,centrifugation 1500rpm/min, serum or plasma as sample.
-Take out the cassette from the foil pouch and place it horizontally.
-Take 10ul serum or plasma into the sample holes “S”.
-And then take Diluent with pipette.
-Gradually drip 3 drops into the hole “S”.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

【Result】
Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.

【Product Display】
