The reagent contains DAN1 antibody one conjugate, DAN2 antibody two conjugate, DAN3 and graphene oxide, Labeled with acridine ester. When the target analyte specifically binds to the antibody, The DNA coupling on the antibody is closer in distance, And then trigger the assembly of three DNA molecules Specific spatial morphology of immune complexes，After adding the exciter, Acridine ester on free DNA3, Due to adsorption by oxidized graphene,The generated light is quenched, And the light generated by acridine ester on the immune complex, Can be detected by PMT. At the same time, it also introduces limitations Endonuclease cycle amplification system, Further improved detection sensitivity.
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